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American Society of Microbiology ABMM Sample Questions:

1. During a primary infection with Epstein-Barr virus (EBV), the virus establishes latency in B lymphocytes.
The transition from lytic to latent infection is tightly regulated by viral gene expression. Which of the following viral proteins is crucial for the establishment and maintenance of EBV latency and is a key target in EBV-associated lymphomas?

A) Early antigen (EA)
B) Glycoprotein gp350
C) Epstein-Barr nuclear antigen 1 (EBNA1)
D) Viral capsid antigen (VCA)

2. A Gram-positive rod is isolated from the blood culture of a neonate with sepsis. The organism exhibits weak beta-hemolysis on sheep blood agar, is catalase-positive, hydrolyzes esculin in the presence of bile, and demonstrates characteristic "tumbling" motility in a wet mount prepared from a broth culture incubated at 25°C. Which organism is most likely?

A) Erysipelothrix rhusiopathiae
B) Bacillus anthracis
C) Corynebacterium jeikeium
D) Listeria monocytogenes

3. A child presents with a characteristic "slapped cheek" rash followed by a lacy, reticular rash on the trunk and extremities. The causative agent is a single-stranded DNA virus belonging to the family Parvoviridae, known as:

A) Measles virus
B) Rubella virus
C) Roseola virus (HHV-6)
D) Human parvovirus B19

4. An elderly patient in a long-term care facility develops a localized outbreak of painful vesicles along a dermatome. Tzanck smear of a vesicle reveals multinucleated giant cells. The MOST likelycausative agent is:

A) Varicella-zoster virus
B) Herpes simplex virus type 1
C) Coxsackievirus
D) Orf virus

5. A microbiology laboratory is performing Gram staining on a bacterial smear. If the safranin counterstain is omitted, Gram-negative bacteria will appear:

A) Brown
B) Colorless
C) Blue
D) Purple

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